Composite

Part:BBa_K5082013:Design

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-15)


pHAGE G3BP1 expression plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2028
    Illegal EcoRI site found at 2972
    Illegal SpeI site found at 2212
    Illegal SpeI site found at 3227
    Illegal PstI site found at 3484
    Illegal PstI site found at 8435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2028
    Illegal EcoRI site found at 2972
    Illegal SpeI site found at 2212
    Illegal SpeI site found at 3227
    Illegal PstI site found at 3484
    Illegal PstI site found at 8435
    Illegal NotI site found at 2879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2028
    Illegal EcoRI site found at 2972
    Illegal BglII site found at 473
    Illegal BglII site found at 4743
    Illegal BglII site found at 4809
    Illegal BglII site found at 4850
    Illegal BamHI site found at 2859
    Illegal XhoI site found at 2871
    Illegal XhoI site found at 8975
    Illegal XhoI site found at 9032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2028
    Illegal EcoRI site found at 2972
    Illegal SpeI site found at 2212
    Illegal SpeI site found at 3227
    Illegal PstI site found at 3484
    Illegal PstI site found at 8435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2028
    Illegal EcoRI site found at 2972
    Illegal SpeI site found at 2212
    Illegal SpeI site found at 3227
    Illegal PstI site found at 3484
    Illegal PstI site found at 8435
    Illegal NgoMIV site found at 1146
    Illegal NgoMIV site found at 4627
    Illegal AgeI site found at 3092
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 455
    Illegal BsaI site found at 4832
    Illegal BsaI site found at 6036
    Illegal BsaI.rc site found at 3982
    Illegal SapI.rc site found at 1107
    Illegal SapI.rc site found at 7118


Design Notes

1


Source

1

References